Nicalin Antibody [mFluor Violet 500 SE]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: mFluor Violet 500 SE

Nicalin Antibody [mFluor Violet 500 SE] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Nicalin
  Met1-Ala522, predicted
  Accession # Q969V3
• Specificity: Detects human, mouse, and rat Nicalin in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: NCLN
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Nicalin Antibody [mFluor Violet 500 SE]

• Ncl-1
• NCLN
• NET59
• nicalin homolog (zebrafish)
• nicalin homolog (zebrefish)
• Nicalin
• Nicastrin-like protein

Background

Nicastrin is an integral part of the Alzheimers associated gamma-secretase complex. Nicalin, a Nicastrin-like protein, is not associated with gamma-secretase but rather interacts with NOMO (Nodal modulator). Nodals are TGFbeta signaling factors that control various cell fate decisions during embryonic body planning stages in vertebrate development. The Nicalin/NOMO complex acts to regulate the Nodal signaling factors during gastrulation. This regulation most often affects the development of the axial mesoderm. Nodal signaling is an important factor for melanoma cell invasiveness and tumorigenicity and inhibition of this signal can promote melanoma cells reverting back toward a melanocyte phenotype.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCLN