Recombinant Human NHP2 Protein

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SDS-Page: Recombinant Human NHP2 Protein [NBP1-50949] - 15 % SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

Order Details

Recombinant Human NHP2 Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-153 of Human NHP2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MTKIKADPDG PEAQAEACSG ERTYQELLVN QNPIAQPLAS RRLTRKLYKC IKKAVKQKQI RRGVKEVQKF VNKGEKGIMV LAGDTLPIEV YCHLPVMCED RNLPYVYIPS KTDLGAAAGS KRPTCVIMVK PHEEYQEAYD ECLEEVQSLP LPL

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
NHP2
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
19.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human NHP2 Protein

  • FLJ20479
  • H/ACA ribonucleoprotein complex subunit 2
  • NHP2 ribonucleoprotein homolog (yeast)
  • NHP2-like protein
  • NHP2P
  • NOLA2member 2 (H/ACA small nucleolar RNPs)
  • Nucleolar protein family A member 2
  • snoRNP protein NHP2

Background

NHP2, also called NOLA2, forms a small ribonucleoprotein particle with GAR1(NOLA1), Nop10 for the isomerization of uridine to pseudouridine. This protein is involved in various aspects of rRNA processing and modification. It localizes to the dense fibrillar component of the nucleolus and in nuclear Cajal bodies. Recombinant human NHP2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol NHP2