NHLRC1 Antibody (S85-18)

Product Discontinued

NHLRC1 Antibody (S85-18) Summary

• Immunogen: Fusion protein amino acids 2-125 (N-terminus encompassing RING domain) of human Malin. 86% identical to rat, and 77% identical to mouse.
• Localization: Endoplasmic Reticulum , Nucleus
• Specificity: Detects approx 42kDa.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NHLRC1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of Malin Antibody was sufficient for detection of malin in 20 ug of transiently (malin) transfected COS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4) and 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for NHLRC1 Antibody (S85-18)

• bA204B7.2
• E3 ubiquitin-protein ligase NHLRC1
• EC 6.3.2.-
• EPM2A
• EPM2BMGC119264
• MALIN
• MGC119262
• MGC119265
• NHL repeat containing 1
• NHL repeat-containing protein 1

Background

The NHLRC1 gene encodes malin, a single subunit E3 ubiquitin (UBB; MIM 191339) ligase, which contains a RING-HC-type zinc finger and 6 NHL domains and is subclassified as a member of the RING-HCa family (Gentry et al., 2005 [PubMed 15930137]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NHLRC1
• Uniprot:
  • NP_940988.2(Human)
  • Q6VVB1(Human)