NHLRC1 Antibody (S85-18)

Images

 
Western Blot: NHLRC1 Antibody (S85-18) [NBP2-42173] - analysis of Monkey COS cells transfected with flag-tagged Malin showing detection of 42 kDa Malin protein using Mouse Anti-Malin Monoclonal Antibody, Clone S85-18. ...read more
Immunocytochemistry/ Immunofluorescence: NHLRC1 Antibody (S85-18) [NBP2-42173] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Malin ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, ICC/IF
Clone
S85-18
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

NHLRC1 Antibody (S85-18) Summary

Immunogen
Fusion protein amino acids 2-125 (N-terminus encompassing RING domain) of human Malin. 86% identical to rat, and 77% identical to mouse.
Localization
Endoplasmic Reticulum, Nucleus
Specificity
Detects approx 42kDa.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NHLRC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunocytochemistry
Application Notes
1 ug/ml of Malin Antibody was sufficient for detection of malin in 20 ug of transiently (malin) transfected COS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 50% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for NHLRC1 Antibody (S85-18)

  • bA204B7.2
  • E3 ubiquitin-protein ligase NHLRC1
  • EC 6.3.2.-
  • EPM2A
  • EPM2BMGC119264
  • MALIN
  • MGC119262
  • MGC119265
  • NHL repeat containing 1
  • NHL repeat-containing protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NHLRC1
Uniprot