NGL-1/LRRC4C Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

NGL-1/LRRC4C Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human NGL‑1/LRRC4C
  Gln45-Lys527
  Accession # Q9HCJ2
• Specificity: Detects human NGL-1/LRRC4C in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: LRRC4C
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NGL-1/LRRC4C Antibody [Biotin]

• KIAA1580NGL-1NGL1Netrin-G1 ligand
• leucine rich repeat containing 4C
• leucine-rich repeat-containing protein 4C
• LRRC4C
• NGL1
• NGL-1

Background

Human NGL-1 (Netrin-G1 ligand) is a 67 kDa (predicted for mature protein), type I transmembrane cell adhesion molecule that is a member of the NGL family of proteins (1, 2). It is synthesized from a precursor that is 640 amino acids (aa) in length that contains a 44 aa signal sequence, a 483 aa extracellular region, a 21 aa transmembrane region, and a short cytoplasmic tail of 92 aa. The extracellular region of NGL-1 consists of nine leucine-rich repeats (LRRs) that are flanked by LRR N-terminal and LRR C-terminal domains, and followed by an Ig-like C2-type domain (1, 2). The cytoplasmic region contains a C-terminal Glu-Thr-Gln-Ile sequence that corresponds to a potential PDZ (postsynaptic density-95/discs large/zona occludens-1) domain-binding motif (1, 2). Human NGL-1 is 99.7% aa identical to mouse NGL-1. Mouse NGL-1 is highly expressed in the developing cerebral cortex and the striatum at embryonic day 14 (1). Postnatally, NGL-1 is expressed exclusively in the brain, with the highest expression found in the cerebral cortex as a whole, and in individual neocortical areas such as the frontal, parietal and occipital lobes (1). Moderate expression of NGL-1 occurs in the putamen, amygdala, hippocampus and medulla oblongata (1). Weak expression is found in the caudate nucleus and thalamus (1). Functionally, membrane-bound cell-surface NGL-1 binds to netrin-G1 specifically through its LRR region, and in the developing brain, may promote neurite outgrowth of thalamocortical axons (1-4). Little is known about NGL-1’s function at later stages.

1. Lin, J.C. et al. (2003) Nat. Neurosci. 6:1270.
2. Kim, S. et al. (2006) Nat. Neurosci. 9:1294.
3. Chen, Y. et al. (2006) Brain Res. Rev. 51:265.
4. Nishimura-Akiyoshi, S. et al. (2007) Proc. Natl. Acad. Sci. U.S.A. 104:14801.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LRRC4C
• Uniprot:
  • Q9HCJ2(Human)