NGL-1/LRRC4C Antibody (755720) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: IHC
• Clone: 755720
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

NGL-1/LRRC4C Antibody (755720) [Alexa Fluor® 594] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human NGL-1/LRRC4C
  Gln45-Lys527 (predicted)
  Accession # Q9HCJ2
• Specificity: Detects human NGL-1/LRRC4C in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) LRRC4 or rhNGL‑3/LRRC4B is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NGL-1/LRRC4C Antibody (755720) [Alexa Fluor® 594]

• KIAA1580NGL-1NGL1Netrin-G1 ligand
• leucine rich repeat containing 4C
• leucine-rich repeat-containing protein 4C
• LRRC4C
• NGL1
• NGL-1

Background

Human NGL-1 (Netrin-G1 ligand; gene name LRRC4C) is a 67 kDa (predicted for mature protein), type I transmembrane cell adhesion molecule that is a member of the NGL family of proteins (1, 2). It is synthesized from a precursor that is 640 amino acids (aa) in length that contains a 44 aa signal sequence, a 483 aa extracellular region, a 21 aa transmembrane region, and a short cytoplasmic tail of 92 aa. The extracellular region of NGL-1 consists of nine leucine-rich repeats (LRRs) that are flanked by LRR N-terminal and LRR C-terminal domains, and followed by an Ig-like C2‑type domain (1, 2). The cytoplasmic region contains a C‑terminal Glu-Thr-Gln-Ile sequence that corresponds to a potential PDZ (postsynaptic density-95/discs large/zona occludens-1) domain-binding motif (1, 2). Human NGL-1 shares 99.7% aa sequence identity with mouse NGL-1. Mouse NGL-1 is highly expressed in the developing cerebral cortex and the striatum at embryonic day 14 (1). Postnatally, NGL-1 is expressed exclusively in the brain, with the highest expression found in the cerebral cortex as a whole, and in individual neocortical areas such as the frontal, parietal and occipital lobes (1). Moderate expression of NGL-1 occurs in the putamen, amygdala, hippocampus and medulla oblongata (1). Weak expression is found in the caudate nucleus and thalamus (1). Functionally, membrane-bound cell-surface NGL-1 binds to netrin-G1 specifically through its LRR region, and in the developing brain, may promote neurite outgrowth of thalamocortical axons (1‑4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.