NFIA Overexpression Lysate (Native)

Images

 
Western Blot: NFIA Overexpression Lysate (Adult Normal) [NBL1-13615] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NFIA.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

NFIA Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204650 and based on accession number NM_005595. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens nuclear factor I/A (NFIA), transcript variant 2, mRNA.
Gene
NFIA

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
54.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for NFIA

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NFIA Overexpression Lysate (Native)

  • CCAAT-box-binding transcription factor
  • CTF
  • DKFZp686J23256
  • FLJ39164
  • KIAA1439DKFZp434L0422
  • NF1-A
  • NF-I/A
  • NFI-A
  • NFI-L
  • nuclear factor 1 A-type
  • Nuclear factor 1/A
  • nuclear factor I/ATGGCA-binding protein

Background

Nuclear factor I (NFI) proteins constitute a family of dimeric DNA-binding proteins with similar, and possibly identical, DNA-binding specificity. They function as cellular transcription factors and as replication factors for adenovirus DNA replication. Diversity in this protein family is generated by multiple genes, differential splicing, and heterodimerization.[supplied by OMIM]. Transcript Variant: This variant (2) lacks an alternate segment in the 3' UTR, which shifts the reading frame, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for NFIA Lysate (NBL1-13615) (0)

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Video Protocols

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Bioinformatics

Gene Symbol NFIA