Neurogranin Antibody - BSA Free

Western Blot: Neurogranin Antibody - BSA Free [NBP1-45219] - Staining of Human Brain (Frontal Cortex) lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Neurogranin Antibody - BSA Free [NBP1-45219] - Staining of paraffin embedded Human Cortex. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Discontinued

Neurogranin Antibody - BSA Free Summary

• Immunogen: Peptide with sequence C-KIKSGERGRKG corresponding to internal region according to NP_006167.1.
• Epitope: internal region
• Specificity: Reported variants represent identical protein: NP_006167.1, NP_001119653.1
• Predicted Species: Mouse (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Clonality: Polyclonal
• Host: Goat
• Gene: NRGN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.1 - 0.3 ug/mL
• Application Notes: WB: Approx. 15 kDa band observed in human brain is reported in scientific literature (PMID: 9880498).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl)
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Reported variants represent identical protein: NP_006167.1, NP_001119653.1

Alternate Names for Neurogranin Antibody - BSA Free

• calmodulin-binding protein
• hng
• neurogranin (protein kinase C substrate, RC3)
• Neurogranin
• Ng
• NRGN
• protein kinase C substrate
• RC3
• RC3ng

Background

Neurogranin (NRGN) is the human homolog of the neuron-specific rat RC3/neurogranin gene. This gene encodes a postsynaptic protein kinase substrate that binds calmodulin in the absence of calcium. The NRGN gene contains four exons and three introns. The exons 1 and 2 encode the protein and exons 3 and 4 contain untranslated sequences. It is suggested that the NRGN is a direct target for thyroid hormone in human brain, and that control of expression of this gene could underlay many of the consequences of hypothyroidism on mental states during development as well as in adult subjects. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neurogranin Antibody (NBP1-45219)(1)

Publication using NBP1-45219

• Ruano D, Aulchenko YS, Macedo A et al. Association of the gene encoding neurogranin with schizophrenia in males. J Psychiatr Res 2008-01-01 [PMID: 17140601]

Bioinformatics

• Gene Symbol: NRGN
• Entrez:
  • 4900(Human)
  • 647011(Mouse)
• Uniprot:
  • NP_006167.1(Human)