Neurexophilin-3 Antibody [Unconjugated]

Western blot shows lysates of human brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Neurexophilin-3/NXPH-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5098) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Neurexophilin-3/NXPH-3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Neurexophilin-3 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat Neurexophilin-3/NXPH-3
  Gln23-Gly252
  Accession # AAI66603
• Specificity: Detects human, mouse, and rat Neurexophilin-3/NXPH-3 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Neurexophilin-1/NXPH1 and recombinant rat Neurexophilin-1/NXPH1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NXPH3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neurexophilin-3 Antibody [Unconjugated]

• KIAA1159
• neurexophilin 3
• Neurexophilin3
• Neurexophilin-3
• NPH3
• NPH3neurexophilin-3
• NXPH3

Background

Neurexophilin-3 (NXPH-3) is a neuropeptide-like secreted glycoprotein in the neurexophilin family (1, 2). The 252 amino acid (aa) NXPH-3 precursor contains a 22 aa signal peptide, plus a 230 aa proprecursor that is likely cleaved at a basic motif, producing a 76 aa propeptide and a 154 aa mature protein (1). Mature rat NXPH-3 shares 99%, 95% and 92% aa identity with mouse, human and bovine NXPH-3, respectively. It contains motifs that are conserved among all neurexophilins, including three potential N-glycosylation sites in the N-terminal portion and six cysteines in the C-terminal portion (1). NXPH-3 is expressed selectively in subplate-derived neurons in the cortex, granule cells in the vestibulocerebellum, and Cajal-Retzius cells during development (3). NXPH-1 is the neurexophilin most similar to NXPH-3, sharing 69% aa identity within the mature region. Expression of NXPH-1 and NXPH-3 does not appear to overlap, with NXPH-1 expression occurring mainly by cells that resemble inhibitory interneurons (2-4). Both are tightly bound extracellular ligands of alpha -neurexins, synaptic transmembrane molecules that are essential for calcium-triggered neurotransmitter release (1, 5, 6). Genetic deletion of NXPH-1 and/or NXPH-3 produces no anatomical effect, although mice lacking NXPH-3 show specific defects in motor coordination (3, 4). Of the other known neurexophilins, NXPH-2 is not expressed in rodents, and NXPH-4 does not bind alpha -neurexins (1, 5).

1. Missler, M. and T. C. Sudhof (1998) J. Neurosci. 18:3630.
2. Petrenko, A. G. et al. (1996) J. Neurosci. 16:4360.
3. Beglopoulos, V. et al. (2005) Mol. Cell. Biol. 25:7278.
4. Clarris, H. J. et al. (2002) Int. J. Dev. Biol. 46:649.
5. Missler, M. et al. (1998) J. Biol. Chem. 273:34716.
6. Dudanova, I. et al. (2006) J. Neurosci. 26:10599.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NXPH3
• Uniprot:
  • AAI66603()