Neprilysin-2/MMEL1 Antibody (353207) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB, IP
• Clone: 353207
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

Neprilysin-2/MMEL1 Antibody (353207) [Alexa Fluor® 532] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Neprilysin-2
  Gly78-Trp779
  Accession # Q495T6
• Specificity: Detects human Neprilysin‑2/MMEL1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Neprilysin, rhECE‑1 or rhECE‑2 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Neprilysin-2/MMEL1 Antibody (353207) [Alexa Fluor® 532]

• EC 3.4.24
• EC 3.4.24.11
• MELL1
• membrane metallo-endopeptidase-like 1
• Membrane metallo-endopeptidase-like 2MGC119455
• MGC119454
• MMEL1
• MMEL2
• NEP2
• NEP2(m)
• NEPIIMGC119456
• Neprilysin II
• Neprilysin2
• Neprilysin-2
• NL2
• NL2NL1
• SEP
• soluble secreted endopeptidase
• zinc metallopeptidase

Background

Neprilysin-2 is a zinc protease of the neprilysin (NEP) family, which also includes NEP, ECE-2, PEX, DINE, Kell and several NEP-like proteins (1). It is encoded by the MMEL1 gene in the human genome and is also known as soluble secreted endopeptidase (SEP), Neprilysin-like 1 and Neprilysin-2 (2). Highly expressed in testis, the cDNA predicted a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Both membrane-bound and soluble forms were comparable with regard to model substrates, pH optima and inhibitor profiles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.