NEI3 Overexpression Lysate (Native)

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Western Blot: NEI3 Overexpression Lysate (Adult Normal) [NBL1-13581] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NEI3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

NEI3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC206838 and based on accession number NM_018248. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens nei endonuclease VIII-like 3 (E. coli) (NEIL3), mRNA.
Gene
NEIL3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
67.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for NEI3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NEI3 Overexpression Lysate (Native)

  • DNA glycosylase FPG2
  • DNA glycosylase hFPG2
  • DNA glycosylase/AP lyase Neil3
  • EC 3.2.2.-
  • EC 4.2.99.18
  • endonuclease 8-like 3
  • Endonuclease VIII-like 3
  • FGP2
  • FLJ10858
  • FPG2
  • hFPG2
  • hNEI3
  • nei endonuclease VIII-like 3 (E. coli)
  • NEI3
  • Nei-like protein 3

Background

NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for NEI3 Lysate (NBL1-13581) (0)

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Video Protocols

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Bioinformatics

Gene Symbol NEIL3