Nectin-4/PVRL4 Antibody [ADC] - (Enfortumab vedotin-ejfv, Research Grade)

Human Nectin-4/PVRL4 MDA-MB-231 LUCI Cell Line were stained with Nectin-4/PVRL4/PVRL4 and negative control protein respectively, washed and then followed by PE and analyzed with FACS,The EC50 is 0.15 mug/mL.

Immobilized human NECTIN 4 His at 2 ug/mL can bind Nectin-4/PVRL4/PVRL4, EC50 is 0.004082 ug/mL.

• Reactivity: Hu
• Applications: ELISA, Flow, Func
• Clonality: Monoclonal
• Host: Human
• Conjugate: ADC
• Concentration: LYOPH

Nectin-4/PVRL4 Antibody [ADC] - (Enfortumab vedotin-ejfv, Research Grade) Summary

• Description: This product is an Antibody-Drug Conjugate (ADC) for Research Use Only (RUO).
  
  Enfortumab vedotin-ejfv is an ADC consisting of a Nectin-4/PVRL4-directed antibody and Monomethyl auristatin E (MMAE) , suitable for adult patients with locally advanced or metastatic urothelial carcinoma, or who have received programmed cell death protein 1 (PD-1) inhibitor or programmed cell death ligand 1 (PD-L1) inhibitor therapy or neoadjuvant/adjuvant platinum-based chemotherapy for locally advanced or metastatic cancer.
  
  This product is shipped at ambient temperature. Upon receipt, store immediately at -20C or lower for 12 months in a lyophilized state. - 80C for 3 months after reconstitution. Avoid repeated freeze-thaw cycles.
• Immunogen: Nectin-4/PVRL4 (Uniprot: Q96NY8)
• Source: CHO
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Human
• Purity: > 90% by SDS-PAGE and HPLC

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Functional
• Theoretical MW: 143.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C in powder form. Store at -80C once reconstituted.
• Buffer: Lyophilized from 25mM histidine, 8% sucrose, 0.01% Tween80 (pH6.2)
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 90% by SDS-PAGE and HPLC
• Reconstitution Instructions: Reconstitute with sterile, distilled water to a final concentration of 1 mg/ml. Gently shake to solubilize completely. Do not vortex.

Alternate Names for Nectin-4/PVRL4 Antibody [ADC] - (Enfortumab vedotin-ejfv, Research Grade)

• EDSS1
• Ig superfamily receptor LNIR
• LNIR
• LNIRnectin 4
• Nectin4
• Nectin-4
• poliovirus receptor-related 4
• poliovirus receptor-related protein 4
• PRR4
• PRR4DKFZp686K05193
• PVRL4

Background

PVRL4, also known as Poliovirus receptor-related protein 4, is a 510 amino acid protein that is 55 kDa, predominantly expressed in placenta and breast carcinoma, is a single-pass type I membrane protein involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with PVRL2/nectin-1, and oes not act as receptor for alpha-herpesvirus entry into cells. Disease research is being performed in relation to this protein and ectodermal dysplasia, syndactyly, ectodermal dysplasia-syndactyly syndrome 1, measles, ovarian cancer, breast carcinoma, lung cancer, and carcinoma. The PVRL4 protein has shown an interaction with PVRL1, MLLT4, PICK1, TIGIT, PARD3, PVRL3, and PVRL2 in Adherens junctions interactions, Nectin/Necl trans heterodimerization, Cell junction organization, Cell-Cell communication, Cell-cell junction organization, Sertoli-Sertoli Cell Junction Dynamics, and Epithelial Adherens Junctions pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.