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Necdin Antibody (OTI5D1) [FITC]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications ICC/IF, IHC
Clone
OTI5D1
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72925AF350
Alexa Fluor 405
NBP2-72925AF405
Alexa Fluor 488
NBP2-72925AF488
Alexa Fluor 532
NBP2-72925AF532
Alexa Fluor 594
NBP2-72925AF594
Alexa Fluor 647
NBP2-72925AF647
Alexa Fluor 700
NBP2-72925AF700
Alexa Fluor 750
NBP2-72925AF750
Biotin
NBP2-72925B
DyLight 350
NBP2-72925UV
DyLight 405
NBP2-72925V
DyLight 488
NBP2-72925G
DyLight 550
NBP2-72925R
DyLight 594
NBP2-72925DL594
DyLight 650
NBP2-72925C
DyLight 680
NBP2-72925FR
DyLight 755
NBP2-72925IR
HRP
NBP2-72925H
Janelia Fluor 549
NBP2-72925JF549
Janelia Fluor 646
NBP2-72925JF646
Unconjugated
NBP2-46117
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46117
Azide and BSA Free
NBP2-72925
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Necdin Antibody (OTI5D1) [FITC] Summary

Immunogen
Full length recombinant protein of human Necdin (NP_002478) produced in HEK293T cells.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
NDN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Necdin Antibody (OTI5D1) [FITC]

  • HsT16328
  • necdin (mouse) homolog
  • necdin homolog (mouse)
  • necdin
  • PWCR

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: SNRPN Antibody [NBP2-20439] - SNRPN antibody detects SNRPN protein at cytosol on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. SNRPN antibody dilution: 1:500.
Western Blot: SNRPN Antibody [NBP2-20439] - Sample (30 ug of whole cell lysate) A: JurKat 12% SDS PAGE gel, diluted at 1:1000.
Species: Hu, Mu, Rt
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Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NRAGE Monoclonal Antibody (Catalog # MAB38352) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NRAGE at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
NRAGE was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human NRAGE Monoclonal Antibody (Catalog # MAB38352) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Immunocytochemistry/Immunofluorescence: MKRN3 Antibody [NBP1-84320] - Staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: MKRN3 Antibody [NBP1-84320] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
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Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-p53 Antibody (PAb 240) NB200-103 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
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[Unconjugated]
292-G2
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Recombinant Human IGF-II/IGF2 (Catalog # 292‑G2) stimulates cell proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 1.5‑6 ng/mL.
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H00007337-M01
Western Blot: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - UBE3A monoclonal antibody (M01), clone 2F6. Analysis of UBE3A expression in PC-12.
Immunocytochemistry/Immunofluorescence: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Analysis of monoclonal antibody to UBE3A on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: E6AP/UBE3A Antibody (2F6) [H00007337-M01] - Analysis of monoclonal antibody to UBE3A on formalin-fixed paraffin-embedded human lung. Antibody concentration 3 ug/ml.
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NGFR/TNFRSF16/p75NTR A ...
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NGF R/TNFRSF16 was detected in immersion fixed canine mesenchymal stem cells using Mouse Anti-Human/Canine NGF R/TNFRSF16 Monoclonal Antibody (Catalog # MAB367) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
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E3-210
RNF4
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Necdin Antibody
(OTI5D1) [FITC]
NBP2-72925F
Necdin Antibody (OTI5D1) [FITC]
Species: Hu
Applications: ICC/IF, IHC

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-72925AF350
Alexa Fluor 405 NBP2-72925AF405
Alexa Fluor 488 NBP2-72925AF488
Alexa Fluor 532 NBP2-72925AF532
Alexa Fluor 594 NBP2-72925AF594
Alexa Fluor 647 NBP2-72925AF647
Alexa Fluor 700 NBP2-72925AF700
Alexa Fluor 750 NBP2-72925AF750
Biotin NBP2-72925B
DyLight 350 NBP2-72925UV
DyLight 405 NBP2-72925V
DyLight 488 NBP2-72925G
DyLight 550 NBP2-72925R
DyLight 594 NBP2-72925DL594
DyLight 650 NBP2-72925C
DyLight 680 NBP2-72925FR
DyLight 755 NBP2-72925IR
FITC NBP2-72925F
HRP NBP2-72925H
Janelia Fluor 549 NBP2-72925JF549
Janelia Fluor 646 NBP2-72925JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol NDN