NDUFB5 Overexpression Lysate (Native)

Images

 
Western Blot: NDUFB5 Overexpression Lysate (Adult Normal) [NBL1-13553] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NDUFB5.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

NDUFB5 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202714 and based on accession number NM_002492. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa (NDUFB5), nuclear gene encoding mitochondrial protein, mRNA.
Gene
NDUFB5

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for NDUFB5

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NDUFB5 Overexpression Lysate (Native)

  • 5 (16kD, SGDH)
  • CISGDH
  • CI-SGDH
  • Complex I-SGDH
  • DKFZp686N02262
  • FLJ30597
  • MGC111204
  • MGC12314
  • NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa
  • NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial
  • SGDH

Background

The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for NDUFB5 Lysate (NBL1-13553). (Showing 1 - 1 of 1 FAQ).

  1. How come the vector transfected control has no band? There should be endogenous level of NDUFB5 in Hek293 cells.
    • We test most of our over-expression lysates with anti-DDK to ensure the protein is at the correct size. NDUFB5 will have endogenous levels in the HEK293 lysate, and you may see a band in the empty vector lane, but you should see an increase in intensity in the over-expression lysate lane.

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Bioinformatics

Gene Symbol NDUFB5