NDUFAF5 Antibody

Images

 
Western Blot: C20orf7 Antibody [NBP2-15644] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:2000.
Immunocytochemistry/ Immunofluorescence: C20orf7 Antibody [NBP2-15644] - SK-N-SH cells were fixed in 2% paraformaldehyde/culture medium at 37 degrees C for 30 min. Green: C20orf7 protein stained by C20orf7 antibody ...read more
Immunohistochemistry-Paraffin: C20orf7 Antibody [NBP2-15644] - Immunohistochemical analysis of paraffin-embedded gastric ca, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NDUFAF5 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human C20orf7. The exact sequence is proprietary.
Localization
Mitochondrion inner membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NDUFAF5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for NDUFAF5 Antibody

  • bA526K24.2
  • C20orf7
  • chromosome 20 open reading frame 7
  • dJ842G6.1
  • EC 2.1.1
  • EC 2.1.1.-
  • FLJ22324
  • MGC90272
  • NADH dehydrogenase (ubiquinone) complex I, assembly factor 5
  • probable methyltransferase C20orf7, mitochondrial

Background

The NADH-ubiquinone oxidoreductase complex (complex I) of the mitochondrial respiratory chain catalyzes the transfer of electrons from NADH to ubiquinone, and consists of at least 43 subunits. The complex is located in the inner mitochondrial membrane. This gene encodes a mitochondrial protein that is associated with the matrix face of the mitochondrial inner membrane and is required for complex I assembly. A mutation in this gene results in mitochondrial complex I deficiency. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NDUFAF5
Uniprot