NDUFA7 Antibody (OTI2G4) - Azide and BSA Free

Images

 
Western Blot: NDUFA7 Antibody (OTI2G4) - Azide and BSA Free [NBP2-72909] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFA7 (Right lane) cDNA for 48 hrs and lysed. Equivalent ...read more
Flow Cytometry: NDUFA7 Antibody (OTI2G4) - Azide and BSA Free [NBP2-72909] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NDUFA7.
Western Blot: NDUFA7 Antibody (OTI2G4) - Azide and BSA Free [NBP2-72909] - Analysis of extracts (35ug) from 9 different cell lines by using anti-NDUFA7 monoclonal antibody.
Flow Cytometry: NDUFA7 Antibody (OTI2G4) - Azide and BSA Free [NBP2-72909] - Analysis of Hela cells, using anti-NDUFA7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: NDUFA7 Antibody (OTI2G4) - Azide and BSA Free [NBP2-72909] - Analysis of Jurkat cells, using anti-NDUFA7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: NDUFA7 Antibody (OTI2G4) - Azide and BSA Free [NBP2-72909] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-NDUFA7 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, CyTOF-ready
Clone
OTI2G4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

NDUFA7 Antibody (OTI2G4) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human NDUFA7 (NP_004992) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NDUFA7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500-2000
Theoretical MW
12.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for NDUFA7 Antibody (OTI2G4) - Azide and BSA Free

  • B14.5a
  • CI-B14.5a
  • complex I B14.5a subunit
  • Complex I-B14.5a
  • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7 (14.5kD, B14.5a)
  • NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7, 14.5kDa
  • NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7
  • NADH-ubiquinone oxidoreductase subunit B14.5a

Background

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for NDUFA7 Antibody (NBP2-72909) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NDUFA7