Recombinant Human NDUFA4 His Protein

SDS-Page: NDUFA4 Recombinant Protein [NBP2-51719] - 15% SDS Page (3 ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human NDUFA4 His Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-81 of Human NDUFA4
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMLRQIIG QAKKHPSLIP LFVFIGTGAT GATLYLLRLA LFNPDVCWDR NNPEPWNKLG PNDQYKFYSV NVDYSKLKKE RPDF

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NDUFA4
• Purity: >80%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 11.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.4M UREA
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >80%, by SDS-PAGE

Alternate Names for Recombinant Human NDUFA4 His Protein

• CI-9k
• CI-MLRQ
• Complex I 9kDa subunit
• Complex I-MLRQ
• FLJ27440
• MGC104422
• MGC126843
• MGC126845
• MLRQ
• NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4 (9kD, MLRQ)
• NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4, 9kDa
• NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4
• NADH-ubiquinone oxidoreductase MLRQ subunit

Background

NDUFA4 is encoded by this gene belongs to the complex I 9kDa subunit family. Mammalian complex I of mitochondrial respiratory chain is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NDUFA4