NDST3 Overexpression Lysate

Western Blot: NDST3 Overexpression Lysate (Adult Normal) [NBL1-13533] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NDST3.

• Reactivity: Hu
• Applications: WB

NDST3 Overexpression Lysate Summary

• Description: Transient overexpression lysate of N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3 (NDST3) The lysate was created in HEK293T cells, using Plasmid ID RC217551 and based on accession number NM_004784. The protein contains a C-MYC/DDK Tag.
• Gene: NDST3

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 100.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NDST3 Overexpression Lysate

• bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 3
• EC 2.8.2.8
• GlcNAc N-deacetylase/ N-sulfotransferase 3
• Glucosaminyl N-deacetylase/N-sulfotransferase 3
• hNDST-3
• HSST3MGC130028
• MGC130029
• N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 3
• NDST-3
• N-heparan sulfate sulfotransferase 3
• N-HSST 3

Background

This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. This monomeric bifunctional enzyme catalyzes the N-deacetylation and N-sulfation of N-acetylglucosamine residues in heparan sulfate and heparin, which are the initial chemical modifications required for the biosynthesis of the functional oligosaccharide sequences that define the specific ligand binding activities of heparan sulfate and heparin. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: NDST3