NCOA5 Antibody

Western Blot: NCOA5 Antibody [NB100-2571] - Detection of Human and Mouse NCOA5 (CIA) on HeLa whole cell lysate using NB100-2571. NCOA5 (CIA) was also immunoprecipitated by rabbit anti-NCOA5 (CIA) antibody NB100-2570.

Immunohistochemistry-Paraffin: NCOA5 Antibody [NB100-2571] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NCOA5 (CIA) used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

NCOA5 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 525 and the C-terminus (residue 579) of human Nuclear Receptor Coactivator 5 (Coactivator Independent of AF-2) using the numbering given in entry NP_066018.1 (GeneID 57727).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCOA5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NCOA5 Antibody

• CIAbA465L10.6
• Coactivator independent of AF-2
• KIAA1637
• NCoA-5
• nuclear receptor coactivator 5

Background

NCOA5 is a nuclear receptor coregulator that can have both coactivator and corepressor functions. It interacts with nuclear receptors for steroids (ESR1 and ESR2) independently of the steroid binding domain (AF-2) of the ESR receptors, and with the orphan nuclear receptor NR1D2. It Is also involved in the coactivation of nuclear steroid receptors (ER) as well as the corepression of MYC/c-myc in response to 17-beta-estradiol (E2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NCOA5
• Entrez:
  • 57727(Human)
  • 228869(Mouse)
• Uniprot:
  • Q9HCD5(Human)