Nav1.5 Knockout HeLa Cell Lysate

Product Discontinued

Nav1.5 Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,-1 bp deletion in exon and 1 bp insertion in exon
• Gene: SCN5A

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for Nav1.5 Knockout HeLa Cell Lysate

• cardiac sodium channel alpha subunit
• cardiac tetrodotoxin-insensitive voltage-dependent sodium channel alpha subunit
• CDCD2VF1
• HB1sodium channel, voltage-gated, type V, alpha (long QT syndrome 3)
• HB2
• HBBD
• HH1CMD1E
• ICCD
• IVF
• LQT3SSS1CMPD2
• Nav1.5
• PFHB1
• SCN5A
• Sodium channel protein cardiac muscle subunit alpha
• sodium channel protein type 5 subunit alpha
• sodium channel protein type V alpha subunit
• Sodium channel protein type V subunit alpha
• sodium channel, voltage-gated, type V, alpha subunit
• Voltage-gated sodium channel subunit alpha Nav1.5

Background

The protein encoded by this gene is an integral membrane protein and tetrodotoxin-resistant voltage-gated sodium channel subunit. This protein is found primarily in cardiac muscle and is responsible for the initial upstroke of the action potential in an electrocardiogram. Defects in this gene are a cause of long QT syndrome type 3 (LQT3), an autosomal dominant cardiac disease. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SCN5A