NAP1L1 Overexpression Lysate (Native)

Images

 
Western Blot: NAP1L1 Overexpression Lysate (Adult Normal) [NBL1-13469] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NAP1L1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

NAP1L1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC220821 and based on accession number NM_004537. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens nucleosome assembly protein 1-like 1 (NAP1L1), transcript variant 2, mRNA.
Gene
NAP1L1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
45.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for NAP1L1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for NAP1L1 Overexpression Lysate (Native)

  • hNRP
  • HSP22-like protein interacting protein
  • MGC23410
  • MGC8688
  • NAP-1 related protein
  • NAP1
  • NAP1LFLJ16112
  • NRPNAP-1-related protein
  • nucleosome assembly protein 1-like 1

Background

This gene encodes a member of the nucleosome assembly protein (NAP) family. This protein participates in DNA replication and may play a role in modulating chromatin formation and contribute to the regulation of cell proliferation. Alternative splicing of this gene results in several transcript variants; however, not all have been fully described. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for NAP1L1 Lysate (NBL1-13469) (0)

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Bioinformatics

Gene Symbol NAP1L1