NAALADase-like 1/NAALADL1 Antibody

Western blot shows lysates of human small intestine. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human NAALADase-like 1/NAALADL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5467) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NAALADase-like 1/NAALADL1 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

NAALADase-like 1/NAALADL1 Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human NAALADase-like 1/NAALADL1
  Ala32-Leu740
  Accession # NP_005459
• Specificity: Detects human NAALADase-like 1/NAALADL1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) NAALADase I, recombinant mouse NAALADase II, and rhNAALADase II is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: NAALADL1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NAALADase-like 1/NAALADL1 Antibody

• I100
• ileal dipeptidylpeptidase
• NAALADase L
• NAALADase like 1
• NAALADASEL
• NAALADase-like 1
• NAALADL1 N-acetylated alpha-linked acidic dipeptidase-like 1
• NAALADL1

Background

NAALADase-like1, also known as NAALADL1, (N-acetylated alpha -linked acidic dipeptidase like 1; also Ileal dipeptidylpeptidase) is a 100-115 kDa member of the peptidase M28 family of enzymes. It is found in the brush border of Ileal epithelium and demonstrates serine protease activity. Human NAALADL1 is a type II transmembrane glycoprotein that is 740 amino acids (aa) in length. It contains an extended extracellular domain (aa 29‑740) that shows a protease-associated region (aa 109‑335) with key catalytic residues at Ser617, Asp657 and His687. Multiple splice variants exist. Two show deletions of aa 161‑201 and 296‑330, respectively, while a third shows a 51 aa insertion after Pro359. Three show 18 to 42 aa substitutions after Pro502, with premature truncations after Ile522, Thr543 and Leu579. A final isoform shows a 17 aa substitution for aa 619‑740. Over aa 32‑740, human NAALADL1 shares 81% aa identity with mouse NAALADL1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAALADL1
• Uniprot:
  • NP_005459()