Myosin Phosphatase [p Thr696] Antibody

Product Discontinued

Myosin Phosphatase [p Thr696] Antibody Summary

• Immunogen: Synthetic phosphopeptide corresponding to residues surroundiing Thr696 of human MYPT1.
• Modification: p Thr696
• Specificity: Recognizes endogenous levels of MYPT1, phosphorylated at (thr696)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPP1R12A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Application Notes: Suitable for use in Western Blot. Other applications not tested.

Reactivity Notes

Human, Monkey, mouse, rat

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM Sodium HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Myosin Phosphatase [p Thr696] Antibody

• MBSmyosin phosphatase, target subunit 1
• MGC133042
• Myosin phosphatase target subunit 1
• Myosin phosphatase-targeting subunit 1
• MYPT1protein phosphatase 1 regulatory subunit 12A
• protein phosphatase 1, regulatory (inhibitor) subunit 12A
• Protein phosphatase myosin-binding subunit

Background

Protein phosphatase 1 (PP1) is a ubiquitous eukaryotic protein serine/threonine phosphatase involved in the regulation of various cell functions. Substrate specificity is determined by the binding of a regulatory subunit to the PP1 catalytic subunit (PP1c). It is estimated that over fifty different regulatory subunits exist (1). The myosin phosphatase holoenzyme is composed of three subunits: the PP1 catalytic subunit (PP1c), a targeting/regulatory subunit (MYPT/myosin-binding subunit of myosin phosphatase) and a 20kD subunit of unknown function (M20). MYPT binding to PP1cd alters the conformation of the catalytic cleft and increases enzyme activity and specificity (2). Two MYPT isoforms that are 61% identical have been described. MYPT1 is widely expressed while MYPT2 expression appears to be exclusive to heart and brain (3). Related family members also include MBS85, MYPT3 and TIMAP (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP1R12A