Myosin Phosphatase Antibody

Immunohistochemistry-Paraffin: Myosin Phosphatase Antibody [NBP1-52478] - Analysis of anti-PPP1R12A / MYPT1 antibody with human skeletal muscle at concentration 3.75 ug/ml.

Flow Cytometry: Myosin Phosphatase Antibody [NBP1-52478] - Myosin Phosphatase antibody flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml).

Immunofluorescence: Myosin Phosphatase Antibody [NBP1-52478] - Myosin Phosphatase antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic and some nuclear staining.

Product Discontinued

Myosin Phosphatase Antibody Summary

• Immunogen: Peptide with sequence C-REDEYKQKYSRTYD, from the C Terminus of the protein sequence according to NP_002471.1, NP_001231919.1, NP_001231921 .1, and NP_001137358.1.
• Specificity: Human Myosin Phosphatase.
• Predicted Species: Hamster (93%), Orangutan (100%), Opossum (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PPP1R12A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA 1:16000
• Application Notes: NBP1-52478 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.

Reactivity Notes

Gorilla, Gibbon, Marmoset. Predicted cross-reactivity based on sequence identity: Rabbit (86%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: TBS (pH 7.3) and 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Myosin Phosphatase Antibody

• MBSmyosin phosphatase, target subunit 1
• MGC133042
• Myosin phosphatase target subunit 1
• Myosin phosphatase-targeting subunit 1
• MYPT1protein phosphatase 1 regulatory subunit 12A
• protein phosphatase 1, regulatory (inhibitor) subunit 12A
• Protein phosphatase myosin-binding subunit

Background

The major protein phosphatase-1 (PP1) is composed of 3 subunits with apparent molecular mass of 110-130, 37 & 20 kDa. The 110-130 kDa component act as a regulatory subunit known as myosine binding (MBS) or myosine phosphatae targeting subunit (MYPT). The N-terminal portion of MYPT binds to both PP1c-delta and phosphorylated myosin, thereby increasing myosin phosphatase activity. Two isoform of MYPT have been identified (MYPT1 & MYPT2) and share 61% sequence identity. While MYPT1 is widely distributed in human tissues, MYPT2 is mostly detected in brain and heart.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP1R12A
• Entrez:
  • 4659(Human)
• Uniprot:
  • O14974(Human)