MYO1H Antibody

Western Blot: MYO1H Antibody [NBP2-26205] - Staining of NIH3T3 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: MYO1H Antibody [NBP2-26205] - (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MYO1H Antibody Summary

• Description: Novus Biologicals Goat MYO1H Antibody (NBP2-26205) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-MYO1H Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EDQVYKDKNGQLR corresponding to C-Terminus according to NP_001158045.1.
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MYO1H
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1-0.3 ug/ml
• Application Notes: WB: Approx. 110 kDa band observed in NIH3T3 lysates (calculated MW of 118 kDa band according to NP_001158045.1). An additional band of unknown identity was also consistently observed at 28 kDa. This band was successfully blocked by incubation with the immunizing peptide. . Preliminary testing was unsuccessful on Rat and Pig Skeletal Muscle for this particular batch.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MYO1H Antibody

• myosin IH
• myosin-1H
• myosin-Ih

Background

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellularmovements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relativeto actin filaments

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MYO1H Antibody (NBP2-26205)(1)

Publication using NBP2-26205

• Tassopoulou-Fishell M, Deeley K, Harvey EM et al. Genetic variation in myosin 1H contributes to mandibular prognathism. Am J Orthod Dentofacial Orthop 2012-01-01 [PMID: 22196185]

Bioinformatics

• Gene Symbol: MYO1H
• Entrez:
  • 283446(Human)
  • 231646(Mouse)
  • 687866(Rat)