MVP Antibody (JM74-73)

Western Blot: MVP Antibody (JM74-73) [NBP2-75579] - Western blot analysis of MVP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: PC-12 cell lysate Lane 3: Hela cell lysate Lane 4: mouse kidney tissue lysate

Immunohistochemistry-Paraffin: MVP Antibody (JM74-73) [NBP2-75579] - Analysis of paraffin-embedded mouse colon tissue using anti-MVP antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: MVP Antibody (JM74-73) [NBP2-75579] - Analysis of paraffin-embedded human colon cancer tissue using anti-MVP antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: MVP Antibody (JM74-73) [NBP2-75579] - Analysis of paraffin-embedded human stomach cancer tissue using anti-MVP antibody. Counter stained with hematoxylin.

Product Discontinued

MVP Antibody (JM74-73) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human MVP aa 13-62 / 893. (SwissProt: Q14764 Human; SwissProt: Q9EQK5 Mouse; SwissProt: Q62667 Rat)
• Localization: Cytoplasm, Nuclear pore complex, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MVP
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 99 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MVP Antibody (JM74-73)

• LRPVAULT1
• Lung resistance-related protein
• major vault protein

Background

MVP encodes the major vault protein which is a lung resistance-related protein. Vaults are multi-subunit structures that may be involved in nucleo-cytoplasmic transport. This protein mediates drug resistance, perhaps via a transport process. It is widely distributed in normal tissues, and overexpressed in multidrug-resistant cancer cells. The protein overexpression is a potentially useful marker of clinical drug resistance. This gene produces two transcripts by using two alternative exon 2 sequences; however, the open reading frames are the same in both transcripts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for MVP Antibody (NBP2-75579). (Showing 1 - 1 of 1 FAQ).

1. Are there any MVP antibodies that will recognize mouse and can be used for immunofluorescence?
   • At this time we do not have an MVP antibody that will recognize mouse and has been used for immunofluorescence. If you would like to try an antibody in an untested species or application you would qualify for our Innovator's Reward program. Through this program if you complete an online review with image, detailing your positive or negative results we will send you a discount voucher for 100% of the purchase price of the reviewed product.

Bioinformatics

• Gene Symbol: MVP