![Immunohistochemistry: Rabbit Muscarinic Acetylcholine Receptor M3/CHRM3 mAb (2D4) [NBP3-15165] - Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody diluted at 1:300 and staining in paraffin-embedded human brain tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.](http://images.novusbio.com/fullsize/nbp3-15165_rabbit-muscarinic-acetylcholine-receptor-m3-chrm3-mab-2d4-immunohistochemistry-1312025162141..jpg "Immunohistochemistry: Rabbit Muscarinic Acetylcholine Receptor M3/CHRM3 mAb (2D4) [NBP3-15165] - Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody diluted at 1:300 and staining in paraffin-embedded human brain tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | ELISA, IHC |
| Clone | 2D4 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (2D4) - BSA Free (NBP3-15165) is a recombinant monoclonal antibody validated for use in IHC and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Additional Information | Recombinant monoclonal antibody expressed in HEK293F cells |
| Immunogen | A synthesized peptide derived from human Muscarinic Acetylcholine Receptor M3/CHRM3. UniProt ID (P20309). |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | CHRM3 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Storage | Store at -20 to -70C. Avoid freeze-thaw cycles. |
| Buffer | PBS, pH 7.4, 150mM NaCl, and 50% glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Affinity purified |
Secondary Antibodies |
Isotype Controls |
Research Areas for Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (NBP3-15165)Find related products by research area.
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| Gene Symbol | CHRM3 |
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