Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (SR1913) - BSA Free

Western Blot: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (SR1913) [NBP3-22212] - Western blot analysis using NBP3-22212 in U87-MG cell lysate.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clone: SR1913
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (SR1913) - BSA Free Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human Muscarinic Acetylcholine Receptor M2/CHRM2 (Uniprot #: P08172)
• Specificity: Detects endogenous levels of total Muscarinic Acetylcholine Receptor M2/CHRM2
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CHRM2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:500-1:2000
• Theoretical MW: 52 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (SR1913) - BSA Free

• 7TM receptor
• cholinergic receptor, muscarinic 2
• CHRM2
• FLJ43243
• HM2
• mAChR M2
• MGC120006
• MGC120007
• muscarinic acetylcholine receptor M2
• muscarinic M2 receptor

Background

Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine. The mAChRs are present in neurons of the central and peripheral nervous systems, cardiac and smooth muscle and various exocrine glands. There are 5 subtypes (m1-m5) of the receptor that have a tissue specific pattern of expression. The m2 receptor is localized primarily in cardiac tissue and is also expressed at low levels in the hippocampus, cortex, striatum, thalamus, basal forebrain, brainstem, lung, vas deferens and uterus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHRM2