MST2/STK3 Antibody

Western Blot: MST2/STK3 Antibody [H00006788-D01P] - Analysis of STK3 expression in A-431.

Immunocytochemistry/Immunofluorescence: MST2/STK3 Antibody [H00006788-D01P] - Analysis of antibody to STK3 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: MST2/STK3 Antibody [H00006788-D01P] - Analysis of STK3 expression in transfected 293T cell line by STK3 polyclonal antibody.Lane 1: STK3 transfected lysate(56.30 KDa).Lane 2: Non-transfected lysate.

Proximity Ligation Assay: MST2/STK3 Antibody [H00006788-D01P] - Analysis of protein-protein interactions between STK3 and CASP3. HeLa cells were stained with anti-STK3 rabbit purified polyclonal 1:1200 and anti-CASP3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Discontinued

MST2/STK3 Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: STK3 (NP_006272.2, 1 a.a. - 491 a.a.) full-length human protein. MEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQRELEEEEENSDEDELDSHTMVKTSVESVGTMRATSTMSEGAQTMIEHNSTMLESDLGTMVINSEDEEEEDGTMKRNATSPQVQRPSFMDYFDKQDFKNKSHENCNQNMHEPFPMSKNVFPDNWKVPQDGDFDFLKNLSLEELQMRLKALDPMMEREIEELRQRYTAKRQPILDAMDAKKRRQQNF
• Specificity: STK3 - serine/threonine kinase 3 (STE20 homolog, yeast),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STK3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MST2/STK3 Antibody

• EC 2.7.11
• FLJ90748
• KRS1
• Mammalian STE20-like protein kinase 2
• Mess1
• MST2
• MST-2
• MST2EC 2.7.11.1
• MST3
• serine/threonine kinase 3 (STE20 homolog, yeast)
• serine/threonine kinase 3 (Ste20, yeast homolog)
• serine/threonine kinase 3
• serine/threonine-protein kinase 3
• Serine/threonine-protein kinase Krs-1
• STE20-like kinase MST2
• STK3

Background

Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (MIM 134638) (Taylor et al., 1996 [PubMed 8816758]; Lee et al., 2001 [PubMed 11278283]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STK3
• Entrez:
  • 6788(Human)
• Uniprot:
  • NP_006272.2(Human)