MSK2 Antibody (RB1020)

Product Discontinued

MSK2 Antibody (RB1020) Summary

• Immunogen: This RSKB (MSK2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-354 amino acids from the C-terminal region of human RSKB (MSK2).
• Localization: Cellular Location Nucleus.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPS6KA4
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 85.606 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.56 mg/ml
• Purity: Ammonium sulfate precipitation

Alternate Names for MSK2 Antibody (RB1020)

• MSK2
• ribosomal protein S6 kinase, 90kDa, polypeptide 4
• RPS6KA4
• RSKB

Background

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK). The calcium/calmodulin-dependent kinase (CAMK) group consists of 75 kinases regulated by Ca2+/CaM and close relative family (CAMK, CAMKL, DAPK, MAPKAPK).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MSK2 Antibody (NBP2-30079)(1)

Publication using NBP2-30079

• Lang E, Bissinger R, Fajol A et al. Accelerated apoptotic death and in vivo turnover of erythrocytes in mice lacking functional mitogen- and stress-activated kinase MSK1/2. Sci Rep. 2015-11-27 [PMID: 26611568] (WB, Human, Mouse)

Bioinformatics

• Gene Symbol: RPS6KA4
• Uniprot:
  • O75676()
  • O75676()