MSK1/RPS6KA5 [p Thr581] Antibody

Product Discontinued

MSK1/RPS6KA5 [p Thr581] Antibody Summary

• Immunogen: Synthetic peptide surrounding phospho-Thr581 of human MSK1.
• Modification: p Thr581
• Localization: Nuclear; predominantly. Cytoplasmic; partially.
• Specificity: This detects 90 kDa MSK 1 only when phosphorylated at Thr581. It does not cross-react with non-phosphorylated MSK 1, or MSK 1 phosphorylated at other sites. It does not cross-react with RSK 1, RSK 2 or RSK 3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPS6KA5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 - 4 ug/ml

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 0.01% BSA, 50% Glycerol
• Preservative: 0.02% Thimerosal
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MSK1/RPS6KA5 [p Thr581] Antibody

• EC 2.7.11
• MGC1911,90 kDa ribosomal protein S6 kinase 5
• MSK1
• MSK1EC 2.7.11.1
• MSPK1
• Nuclear mitogen- and stress-activated protein kinase 1
• ribosomal protein S6 kinase alpha-5
• ribosomal protein S6 kinase, 90kD, polypeptide 5
• ribosomal protein S6 kinase, 90kDa, polypeptide 5
• RLPK
• RPS6KA5
• RSKL
• RSK-like protein kinase
• S6K-alpha-5

Background

MSK 1, a Mitogen and Stress activated protein Kinase, is activated by ERK as well as p38 MAPK in response to growth factors and various cellular stress stimuli. MSK 1 resembles RSK by having two kinase domains connected by a regulator linker region. Phosphorylation of RSK 1 at Ser 364, Ser381 and Thr574 is critical for RSK 1 activity. These sites are analogous to Ser360, Ser376 and Thr581 of MSK 1 and maybe important for MSK 1 activity as well.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MSK1/RPS6KA5 Antibody (NB120-2562)(1)

Publication using NB120-2562

• Zhang J, Guo L, Zhang Q et al. Aloe emodin suppresses EGF‑induced neoplastic cell transformation by inhibiting the ERk/MSk1 and AkT/GSk3B signaling pathways. Mol Med Rep. 2018-09-27 [PMID: 30272294] (WB, Mouse)

Bioinformatics

• Gene Symbol: RPS6KA5
• Entrez:
  • 9252(Human)
  • 73086(Mouse)
• Uniprot:
  • O75582()