MRPS27 Antibody (OTI1A2) - Azide and BSA Free

Western Blot: MRPS27 Antibody (OTI1A2) - Azide and BSA Free [NBP2-72784] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.

Immunohistochemistry: MRPS27 Antibody (OTI1A2) - Azide and BSA Free [NBP2-72784] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MRPS27 mouse monoclonal antibody.

Flow Cytometry: MRPS27 Antibody (OTI1A2) - Azide and BSA Free [NBP2-72784] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-MRPS27 antibody, and then analyzed by flow cytometry.

Immunohistochemistry: MRPS27 Antibody (OTI1A2) - Azide and BSA Free [NBP2-72784] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MRPS27 mouse monoclonal antibody.

Immunohistochemistry: MRPS27 Antibody (OTI1A2) - Azide and BSA Free [NBP2-72784] - Staining of paraffin-embedded Human lung tissue using anti-MRPS27 mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: OTI1A2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

MRPS27 Antibody (OTI1A2) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse MRPS27 Antibody (OTI1A2) - Azide and BSA Free (NBP2-03888) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 151-414 of human MRPS27 (NP_055899) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MRPS27
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 47.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for MRPS27 Antibody (OTI1A2) - Azide and BSA Free

• FLJ21764
• KIAA0264FLJ23348
• mitochondrial ribosomal protein S27
• mitochondrial
• MRP-S27

Background

MRPS27, or Mitochondrial Ribosomal Protein S27, consists of a 414 amino acid isoform that is 48 kDa, and is involved in the manufacture of proteins as a portion of the small 28S mitochondrial ribosomal subunit. MRPS27 is not currently involved in any disease research. The protein is not linked to any pathways, but does interact with several other proteins, including YWHAZ, YWHAQ, HNRNPA1, ICT1, and DHX30.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRPS27