MRPS27 Antibody (OTI1A2)

Western Blot: MRPS27 Antibody (1A2) [NBP2-03888] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MRPS27 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRPS27.

Immunohistochemistry-Paraffin: MRPS27 Antibody (1A2) [NBP2-03888] Staining of paraffin-embedded Human lung tissue using anti-MRPS27 mouse monoclonal antibody.

Flow Cytometry: MRPS27 Antibody (1A2) [NBP2-03888] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MRPS27 antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: MRPS27 Antibody (1A2) [NBP2-03888] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-MRPS27 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: MRPS27 Antibody (1A2) [NBP2-03888] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MRPS27 mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, Flow, IHC
• Clone: OTI1A2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

MRPS27 Antibody (OTI1A2) Summary

• Description: Novus Biologicals Mouse MRPS27 Antibody (OTI1A2) (NBP2-03888) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 151-414 of human MRPS27 (NP_055899) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MRPS27
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 47.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MRPS27 Antibody (OTI1A2)

• FLJ21764
• KIAA0264FLJ23348
• mitochondrial ribosomal protein S27
• mitochondrial
• MRP-S27

Background

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). [provided by RefSeq]. COMPLETENESS: complete on the 3' end.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRPS27
• Entrez:
  • 23107(Human)