Mouse DPPIV/CD26 DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse DPPIV/CD26 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Dpp4

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse DPPIV/CD26 DuoSet ELISA, 15 Plate

• ADABP
• ADCP-2
• ADCP2DPP IV
• Adenosine deaminase complexing protein 2TP103
• CD26 antigen
• CD26
• CD26T-cell activation antigen CD26
• dipeptidyl peptidase 4
• Dipeptidyl peptidase IV
• dipeptidylpeptidase 4
• dipeptidyl-peptidase 4
• dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
• DPP4
• DPPIV
• EC 3.4.14.5

Background

DPPIV/CD26 is a serine exopeptidase that is expressed as a noncovalent homodimer on the surface of epithelial cells, endothelial cells, and activated lymphocytes. It regulates multiple aspects of immune and endocrine function by cleaving Xaa-Pro or Xaa-Ala dipeptides from the N-terminus of a wide variety of chemokines (CCL4 and 5, CXCL6, 9, 10, 11, and 12), growth factors (GM-CSF, IL-3), and peptide hormones (Glucagon, Glucagon-like Peptides 1 and 2, GIP, GHRH, Procalcitonin, Neuropeptide Y, and Substance P). DPPIV interacts in cis with Adenosine Deaminase on T cells, in trans with Caveolin-1 on antigen presenting cells, and it serves as a cell entry coreceptor for HIV and coronavirus. It provides costimulatory proliferation and activation signals to both CD4+ and CD8+ T cells. A soluble form of DPPIV can be proteolytically shed from adipocytes, leading to insulin resistance in adipocytes and skeletal muscle.

Publications for DPPIV/CD26 (DY954)(8)

Publications using DY954

• NA Trzaskalsk, B Vulesevic, MA Nguyen, N Jeraj, E Fadzeyeva, NM Morrow, CA Locatelli, N Travis, AA Hanson, JR Nunes, C O'Dwyer, JN Van der Ve, I Lorenzen-S, R Seymour, SM Pulente, AC Clément, AM Crawley, RL Jacobs, MA Doyle, CL Cooper, KH Kim, MD Fullerton, EE Mulvihill Hepatocyte-derived DPP4 regulates portal GLP-1 bioactivity, glucose production, and its absence alters liver disease progression JCI Insight, 2023-01-24;0(0):. 2023-01-24 [PMID: 36472923] (Mouse)
• R Kamakura, GS Raza, A Prasannan, J Walkowiak, KH Herzig Dipeptidyl peptidase 4 and GLP-1 interplay in STC-1 and GLUTag cell lines Peptides, 2020-09-28;0(0):170419. 2020-09-28 [PMID: 32998057] (Mouse)
• LL Baggio, EM Varin, JA Koehler, X Cao, Y Lokhnygina, SR Stevens, RR Holman, DJ Drucker Plasma levels of DPP4 activity and sDPP4 are dissociated from inflammation in mice and humans Nat Commun, 2020-07-28;11(1):3766. 2020-07-28 [PMID: 32724076] (Mouse)
• S Saussentha, M Ouni, C Baumeier, K Schwerbel, P Gottmann, S Christmann, T Laeger, A Schürmann Epigenetic regulation of hepatic Dpp4 expression in response to dietary protein J. Nutr. Biochem., 2018-10-13;63(0):109-116. 2018-10-13 [PMID: 30359860] (Mouse)
• M Rödiger, MW Werno, I Wilhelmi, C Baumeier, D Hesse, N Wettschure, S Offermanns, K Song, M Krau beta , A Schürmann Adiponectin release and insulin receptor targeting share trans-Golgi-dependent endosomal trafficking routes Mol Metab, 2017-11-22;0(0):. 2017-11-22 [PMID: 29203237] (Mouse)
• C Baumeier, L Schlüter, S Saussentha, T Laeger, M Rödiger, SA Alaze, L Fritsche, HU Häring, N Stefan, A Fritsche, RW Schwenk, A Schürmann Elevated hepatic DPP4 activity promotes insulin resistance and non-alcoholic fatty liver disease Mol Metab, 2017-08-04;6(10):1254-1263. 2017-08-04 [PMID: 29031724] (Mouse)
• Barreira da Silva R, Laird M, Yatim N, Fiette L, Ingersoll M, Albert M Dipeptidylpeptidase 4 inhibition enhances lymphocyte trafficking, improving both naturally occurring tumor immunity and immunotherapy. Nat Immunol, 2015-06-15;16(8):850-8. 2015-06-15 [PMID: 26075911] (Mouse)
• Pang , Zhengda, Nakagami , Hironori, Osako , Mariana, Koriyama , Hiroshi, Nakagami , Futoshi, Tomioka , Hideki, Shimamura , Munehisa, Kurinami , Hitomi, Takami , Yoichi, Morishita , Ryuichi, Rakugi , Hiromi Therapeutic vaccine against DPP4 improves glucose metabolism in mice. Proc Natl Acad Sci U S A, 2014-03-17;111(13):E1256-63. 2014-03-17 [PMID: 24639549] (Mouse)

FAQs for DPPIV/CD26 (DY954). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Dpp4