MMADHC Antibody - BSA Free

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Western Blot: MMADHC Antibody [NBP1-54708] - Human Muscle lysate, concentration 0.2-1 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
0.5 mg/ml

Order Details

MMADHC Antibody - BSA Free Summary

Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Immunogen
Synthetic peptides corresponding to MMADHC. The peptide sequence was selected from the middle region of MMADHC (NP_056517). Peptide sequence RAEGYWADFIDPSSGLAFFGPYTNNTLFETDERYRHLGFSVDDLGCCKVI. The peptide sequence for this immunogen was taken from within the described region.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MMADHC
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1.0 ug/ml
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for MMADHC Antibody - BSA Free

  • cblD
  • chromosome 2 open reading frame 25
  • methylmalonic aciduria (cobalamin deficiency) cblD type, with homocystinuria
  • mitochondrial
  • protein C2orf25, mitochondrial

Background

The function of C2orf25 remains unknown.Vitamin B12 (cobalamin) is an essential cofactor in several metabolic pathways. Intracellular conversion of cobalamin to adenosylcobalamin in mitochondria and to methylcobalamin in cytoplasm is necessary for homeostasis of methylmalonic acid and homocysteine. C2ORF25 encodes a protein involved in an early step of cobalamin metabolism (Coelho et al., 2008 [PubMed 18385497]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol

FAQs for MMADHC Antibody (NBP1-54708). (Showing 1 - 1 of 1 FAQ).

  1. My questions is about product number NBP1-54708. I'm trying to find information about what secondary antibody to use against this product. What secondary antibody was used in the Western blot associated with the antibody? Also, would you be able to tell me the method of detection for the Western blot as well (i.e. chemiluminescence or colorimetric)?
    • The secondary antibody used in the Western blot was goat-anti-rabbit-HRP at a dilution of 1:5000 and the detection method used was chemiluminescence.

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Bioinformatics

Gene Symbol MMADHC
Entrez
Uniprot