MKRN2 Antibody (5F8) - Azide and BSA Free

Western Blot: MKRN2 Antibody (5F8) [H00023609-M01] - MKRN2 monoclonal antibody (M01), clone 5F8 Analysis of MKRN2 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: MKRN2 Antibody (5F8) [H00023609-M01] - Analysis of monoclonal antibody to MKRN2 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: MKRN2 Antibody (5F8) [H00023609-M01] - Analysis of MKRN2 over-expressed 293 cell line, cotransfected with MKRN2 Validated Chimera RNAi ( Cat # H00023609-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MKRN2 monoclonal antibody (M01), clone 5F8 (Cat # H00023609-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: MKRN2 Antibody (5F8) [H00023609-M01] - Analysis of MKRN2 expression in transfected 293T cell line by MKRN2 monoclonal antibody (M01), clone 5F8.Lane 1: MKRN2 transfected lysate(46.9 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: MKRN2 Antibody (5F8) [H00023609-M01] - Detection limit for recombinant GST tagged MKRN2 is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, KD
• Clone: 5F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MKRN2 Antibody (5F8) - Azide and BSA Free Summary

• Immunogen: MKRN2 (NP_054879, 317 a.a. ~ 415 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AFKQGMGKKACKYFEQGKGTCPFGSKCLYRHAYPDGRLAEPEKPRKQLSSQGTVRFFNSVRLWDFIENRESRHVPNNEDVDMTELGDLFMHLSGVESSE
• Specificity: MKRN2 - makorin, ring finger protein, 2
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MKRN2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, RNAi validation and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MKRN2 Antibody (5F8) - Azide and BSA Free

• EC 6.3.2.-
• HSPC070
• makorin ring finger protein 2
• probable E3 ubiquitin-protein ligase makorin-2
• RNF62RING finger protein 62

Background

Members of the makorin family, including MKRN2, have a characteristic zinc finger composition that suggests that they are ribonucleoproteins (Gray et al., 2001 [PubMed 11597136]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MKRN2
• Entrez:
  • 23609(Human)
• OMIM:
  • 608426(Human)
• Uniprot:
  • NP_054879(Human)