MKRN2 Antibody (5F8)

Images

 
Western Blot: MKRN2 Antibody (5F8) [H00023609-M01] - MKRN2 monoclonal antibody (M01), clone 5F8 Analysis of MKRN2 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: MKRN2 Antibody (5F8) [H00023609-M01] - Analysis of monoclonal antibody to MKRN2 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: MKRN2 Antibody (5F8) [H00023609-M01] - Analysis of MKRN2 over-expressed 293 cell line, cotransfected with MKRN2 Validated Chimera RNAi ( Cat # H00023609-R01V ) (Lane 2) or non-transfected control (Lane 1). ...read more
Western Blot: MKRN2 Antibody (5F8) [H00023609-M01] - Analysis of MKRN2 expression in transfected 293T cell line by MKRN2 monoclonal antibody (M01), clone 5F8.Lane 1: MKRN2 transfected lysate(46.9 KDa).Lane 2: ...read more
Sandwich ELISA: MKRN2 Antibody (5F8) [H00023609-M01] - Detection limit for recombinant GST tagged MKRN2 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
5F8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

MKRN2 Antibody (5F8) Summary

Immunogen
MKRN2 (NP_054879, 317 a.a. ~ 416 a.a) partial recombinant protein with GST tag.AFKQGMGKKACKYFEQGKGTCPFGSKCLYRHAYPDGRLAEPEKPRKQLSSQGTVRFFNSVRLWDFIENRESRHVPNNEDVDMTELGDLFMHLSGVESSE
Specificity
MKRN2 - makorin, ring finger protein, 2
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MKRN2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, RNAi validation and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MKRN2 Antibody (5F8)

  • EC 6.3.2.-
  • HSPC070
  • makorin ring finger protein 2
  • probable E3 ubiquitin-protein ligase makorin-2
  • RNF62RING finger protein 62

Background

Members of the makorin family, including MKRN2, have a characteristic zinc finger composition that suggests that they are ribonucleoproteins (Gray et al., 2001 [PubMed 11597136]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MKRN2
Entrez
OMIM
Uniprot