MKRN1 Antibody

Western Blot: MKRN1 Antibody [NBP2-27545] - Staining of Mouse fetal (A) and adult (B) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: MKRN1 Antibody [NBP2-27545] - HEK293 lysate (10ug protein in RIPA buffer) over expressing Human MKRN1 with DYKDDDDK tag probed with NBP2-27545 (0.3ug/ml) in Lane A and probed with anti- DYKDDDDK Tag (1:1000) in lane C. Mock-transfected HEK293 probed with NBP2-27545 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MKRN1 Antibody Summary

• Description: Novus Biologicals Goat MKRN1 Antibody (NBP2-27545) is a polyclonal antibody validated for use in WB and ELISA. Anti-MKRN1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RYEHSKPLKQEEAT corresponding to internal region according to NP_038474.2, NP_001138597.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_038474.2; NP_001138597.1).
• Predicted Species: Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MKRN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in fetal mouse brain lysates and 35 kDa band plus fainter 50 kDa band bands in adult mouse brain lysates (calculated MW of 52.9 kDa band according to NP_061280.2). The observed 35 kDa band corresponds to the calculated MW of human isoform 2 (35.2 kDa band according to NP_001138597.1), but this size isoform has not yet been confirmed in mouse. In transfected HEK293 transiently expressing full-length human MKRN1 (myc and DYKDDDDK tagged), a band of approx. 60 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MKRN1 Antibody

• E3 ubiquitin-protein ligase makorin-1
• EC 6.3.2.-
• FLJ21334
• makorin ring finger protein 1
• RNF61RING finger protein 61

Background

MKRN1 was identified as a repressor of c-Jun, androgen receptor, and retinoic acid receptor transcriptional activity. MKRN1 is a RING zinc finger protein that has been demonstrated to function as an E3 ubiquitin ligase. The repressive activity of MKRN1 is not dependent on its ubiquitin ligase activity. MKRN1 also appears to function as a transactivator of RNA polymerase II dependent transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MKRN1 Antibody (NBP2-27545)(1)

Publication using NBP2-27545

• Kim JH, Park SM, Kang MR et al. Ubiquitin ligase MKRN1 modulates telomere length homeostasis through a proteolysis of hTERT. Genes Dev 2005-04-01 [PMID: 15805468]

Bioinformatics

• Gene Symbol: MKRN1
• Entrez:
  • 54484(Mouse)
• Uniprot:
  • NP_038474.2(Human)