Miz-1/ZBTB17 Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 647

Miz-1/ZBTB17 Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human Miz-1
  Met1-Ala188
  Accession # Q13105
• Specificity: Detects endogenous human Miz-1 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Miz-1/ZBTB17 Antibody [Alexa Fluor® 647]

• MIZ1
• Miz-1
• Myc-interacting zinc finger protein 1
• mZ13
• pHZ-67
• ZBTB17
• Zfp100
• zinc finger and BTB domain containing 17
• zinc finger and BTB domain-containing protein 17
• zinc finger protein 151 (pHZ-67)
• Zinc finger protein 151
• Zinc finger protein 60MIZ-1
• ZNF151
• ZNF151Myc-interacting Zn finger protein-1
• ZNF60

Background

Miz-1 (Myc-interacting zinc finger protein 1; also ZBTB17) is an 88 kDa, DNA-binding protein that belongs to the Kruppel C2H2-type zinc finger protein family. Human Miz-1 is 803 amino acids (aa) in length, and contains a POZ domain (aa 1 - 104), two Myc-interaction segments (aa 269 - 308 & 637 - 718), and 12 C2H2-type zinc finger motifs (aa 306 - 637); also, there is one alternate splice form that shows an eight aa substitution for Thr769. Miz-1 induces cell arrest at G₁, an effect mediated by its activation of the gene coding for p15^INK4b. This effect is blocked by Myc, which displaces transcriptional coactivators bound to Miz-1. Notably, although the downregulation of Miz-1 may contribute to Myc-induced cell transformation, the deactivation of Miz-1 is absolutely essential for Myc-induced apoptosis. Over aa 1‑188, human Miz-1 is 85% aa identical to mouse Miz-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.