MHC Class II Antibody (HIS19) [FITC]

Images

 
Flow Cytometry: MHC Class II Antibody (HIS19) [FITC] [NBP1-43985] - Staining of rat splenocytes with Anti-Rat MHC Class II FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow
Clone
HIS19
Clonality
Monoclonal
Host
Mouse
Concentration
0.5 mg/ml

MHC Class II Antibody (HIS19) [FITC] Summary

Immunogen
The immunogen for this antibody is MHC Class II.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HLA-DMA
Purity
Protein A or G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
FITC
Format
FITC (A=495, E=519)
Buffer
Aqueous buffer, may contain carrier protein/stabilizer (proprietary)
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
Application Notes
The HIS19 antibody has been tested by flow cytometric analysis of rat spleen or lymph node cell suspensions. This can be used at less than or equal to 0.5 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 uL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This HIS19 clone has also been reported for use in immunoprecipitation and immunohistochemical staining of frozen tissue sections. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Reactivity Notes

Rat

Alternate Names for MHC Class II Antibody (HIS19) [FITC]

  • class II histocompatibility antigen, M alpha chain
  • D6S222E
  • DMA
  • HLA class II histocompatibility antigen, DM alpha chain
  • major histocompatibility complex, class II, DM alpha
  • MHC class II antigen DMA
  • Really interesting new gene 6 protein
  • RING6HLADM

Background

The HIS19 monoclonal antibody reacts with a polymorphic epitope of the rat MHC class II molecule, RT1B. This antibody reacts with all rat haplotypes except the RT1n (including BN and MAXX strains). The rat MHC class II (I-A equivalent), RT1B, is expressed by antigen presenting cells including B cells, dendritic cells and macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Blogs on MHC Class II.

CD74 - a central player in antigen presentation by MHC class II
Cluster of differentiation 74 (CD74) is an important integral membrane protein that serves as a chaperone for MHC class II molecules. CD74, also known as the invariant chain or Ii, is needed for the proper folding and trafficking of MHC class II in...  Read full blog post.

Lymphocyte activation gene 3 (LAG3): No lag time in immune response
The LAG3 protein belongs to the Ig superfamily and contains 4 extracellular Ig-like domains (D1-D4). This molecule plays an key role in the immune response through negative regulation of T-cell proliferation, function, and homeostasis. It is required...  Read full blog post.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol HLA-DMA

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