Human MGMT ELISA Kit (Colorimetric)

ELISA: Human MGMT ELISA Kit (Colorimetric) [NBP2-60580] - Standard curve.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell Culture
• Standard Curve Range: 15.625 - 1000 ng/mL (example only; lot dependent)
• Sensitivity: 4.5 ng/mL (example only; lot dependent)

Human MGMT ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 15.625 - 1000 ng/mL (example only; lot dependent)
• Sensitivity: 4.5 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 11.2% (example only; lot dependent)
• Intra-Assay: %CV < 6.1% (example only; lot dependent)
• Spike Recovery: 89 - 117% (example only; lot dependent)
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: MGMT

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human MGMT ELISA Kit (Colorimetric)

• 6-O-methylguanine-DNA methyltransferase
• EC 2.1.1.63
• methylated-DNA--protein-cysteine methyltransferase
• methylguanine-DNA methyltransferase
• MGMT
• O-6-methylguanine-DNA methyltransferase
• O6-methylguanine-DNA methyltransferase
• O-6-methylguanine-DNA-alkyltransferase

Background

O6-Methylguanine-DNA Methyltransferase (MGMT) is an important DNA repair protein involved in tumor cell resistance to the cytotoxic activity of chemotherapeutic alkylating agents. This protein is also effective in protecting normal cells against the genotoxic and carcinogenic effects of DNA alkylation. The alkylating drug resistance is caused by MGMT's ability to remove DNA alkyl groups introduced in the O6 position of guanine. MGMT is expressed in highly variable amounts, depending upon the cell and tissue type, species, and cellular growth characteristics. In addition, MGMT activity varies among groups of tumors and within a particular type of tumor.

MGMT antibodes are useful tools for DNA repair studies and for some cancer research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Reviews for MGMT ELISA Kit (NBP2-60580) (2)

Average Rating: 5

(Based on 2 reviews)

reviewed by: Verified Customer 10/14/2022

Summary

• Sample Tested: Cancer cell lysates
• Lot: 20101025

Comments

• Comments: The Human MGMT ELISA was used to assess MGMT expression in Temozolomide-Resistant versus parental glioma lines. Results were very consistent.

reviewed by: MARINA KOUTSIOUMPA 04/18/2022

Summary

• Sample Tested: Human Glioma cells, whole cell lysates
• Lot: 20101025

FAQs for MGMT ELISA Kit (NBP2-60580). (Showing 1 - 1 of 1 FAQ).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: MGMT