MEPCE Antibody

Western Blot: MEPCE Antibody [NBP1-28464] - (0.3ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MEPCE Antibody Summary

• Immunogen: Peptide with sequence C-TPHNTSKGFQRP corresponding to internal region according to NP_062552.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MEPCE
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 75 kDa band observed in K562 lysates (calculated MW of 74.4 kDa band according to NP_062552.2).
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MEPCE Antibody

• BCDIN37SK snRNA methylphosphate capping enzyme
• bicoid-interacting 3, homolog
• Bin3 homolog
• bin3, bicoid-interacting 3, homolog (Drosophila)
• EC 2.1.1.-
• FLJ20257
• methylphosphate capping enzyme

Background

MEPCE belongs to the methyltransferase superfamily and contains one BIN3 domain. It is an S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it. MEPCE is expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. It is weakly or not expressed in other tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MEPCE
• Entrez:
  • 56257(Human)
• Uniprot:
  • Q7L2J0(Human)