Human Melanoma Whole Tissue Lysate (Adult Whole Tumor)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Concentration
5.0 mg/ml

Order Details

Human Melanoma Whole Tissue Lysate (Adult Whole Tumor) Summary

Specificity
Total Protein - Human Tumor Tissue: Melanoma
Gene
CDKN2A

Applications/Dilutions

Dilutions
  • Immunoprecipitation
  • Western Blot
Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Concentration
5.0 mg/ml

Lysate Details for Array

Type
Tissue
Life Stage
Adult
Tissue
Skin
Protein State
Native
Tissue Condition
Tumor
Subcellular Fraction
Whole

Notes

We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use.

Alternate Names for Human Melanoma Whole Tissue Lysate (Adult Whole Tumor)

  • ARF
  • CDK4 inhibitor p16-INK4
  • CDK4IP14ARF
  • CDKN2
  • cell cycle negative regulator beta
  • CMM2P16-INK4A
  • Cyclin-dependent kinase 4 inhibitor A
  • cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
  • cyclin-dependent kinase inhibitor 2A
  • INK4
  • INK4A
  • MLMP16INK4
  • MTS-1
  • MTS1P14
  • Multiple tumor suppressor 1
  • p14
  • p14ARF
  • P16
  • p16-INK4
  • P16INK4A
  • p16-INK4a
  • P19
  • P19ARF
  • TP16

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Applications: WB, IP

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Video Protocols

WB Video Protocol

FAQs for Melanoma Lysate (NB820-59436) (0)

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Bioinformatics

Gene Symbol CDKN2A