MEK5 Antibody (SD208-6)

Western Blot: MEK5 Antibody (SD208-6) [NBP2-67763] - Western blot analysis of MEK5 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabb

Immunocytochemistry/Immunofluorescence: MEK5 Antibody (SD208-6) [NBP2-67763] - Staining MEK5 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Western Blot: MEK5 Antibody (SD208-6) [NBP2-67763] - Analysis of MEK5 on Hela cells lysates using anti-MEK5 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: MEK5 Antibody (SD208-6) [NBP2-67763] - Staining MEK5 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: MEK5 Antibody (SD208-6) [NBP2-67763] - Staining MEK5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

MEK5 Antibody (SD208-6) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human MEK5 aa 381-330 / 448. (SwissProt: Q13163 Human)
• Localization: Spindle, Cytosol, nucleus, cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAP2K5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MEK5 Antibody (SD208-6)

• HsT17454
• MAP kinase kinase 5
• MAP kinase kinase MEK5b
• MAP2K5
• MAPK/ERK kinase 5
• MAPKK 5
• MAPKK5
• MAPKK5EC 2.7.12.2
• MEK5
• MEK5MEK 5
• mitogen-activated protein kinase kinase 5
• MKK5
• PRKMK5
• PRKMK5dual specificity mitogen-activated protein kinase kinase 5

Background

Mitogen-acitvated protein kinase kinase 5 (MKK5) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase (1-2). The MAPK cascade plays an important role in signaling defense responses. Of the MKK family members, MKK5 exhibits the closest homology to MEK1 and MEK2, with 46% amino acid identity in the catalytic domain (3) MKK has been show to activate extracellular-signal-regulated protein kinase 5 (ERK5). Also, activated ERK5 phosphorylates mitogen- MKK5 extensively at Ser129, Ser137, Ser142 and Ser149, which are located within the region in MKK5 that is thought to interact with ERK5 (3). There have also been findings to that the MEK5 (MKK5)/ERK5 (BMK) pathway may play a role in prostate cancer (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAP2K5