MEK1 [p Ser298] Antibody (SD206-7)

Western Blot: MEK1 [p Ser298] Antibody (SD206-7) [NBP2-67770] - Analysis of Phospho-MEK1 (S298) on different lysates using anti-Phospho-MEK1 (S298) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: A431

Immunocytochemistry/Immunofluorescence: MEK1 [p Ser298] Antibody (SD206-7) [NBP2-67770] - Staining Phospho-MEK1 (S298) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MEK1 [p Ser298] Antibody (SD206-7) [NBP2-67770] - Analysis of paraffin-embedded mouse colon tissue using MEK1 [p Ser298] antibody. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: MEK1 [p Ser298] Antibody (SD206-7) [NBP2-67770] - Staining Phospho-MEK1 (S298) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: MEK1 [p Ser298] Antibody (SD206-7) [NBP2-67770] - Staining Phospho-MEK1 (S298) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MEK1 [p Ser298] Antibody (SD206-7) [NBP2-67770] - Analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-MEK1 (S298) antibody. Counter stained with hematoxylin.

Product Discontinued

MEK1 [p Ser298] Antibody (SD206-7) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser298 of Human MEK1aa 271-320 / 393. (SwissProt: Q02750 Human; SwissProt: P31938 Mouse; SwissProt: Q01986 Rat)
• Modification: p Ser298
• Localization: Cytoplasm, Nucleus, Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAP2K1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MEK1 [p Ser298] Antibody (SD206-7)

• dual specificity mitogen-activated protein kinase kinase 1
• ERK activator kinase 1
• MAP kinase kinase 1
• MAP2K1
• MAPK/ERK kinase 1
• MAPKK1
• MEK1
• MEK1MAPKK 1
• mitogen-activated protein kinase kinase 1
• MKK1
• MKK1MEK 1
• PRKMK1EC 2.7.12.2
• protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Background

MEK 1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). This pathway also plays a key role in synaptic plasticity in brain (Adams and Sweatt, 2002). Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992) Conversely there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386 (Mansour et al., 1994).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for MEK1 Antibody (NBP2-67770) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-P Mouse 03/11/2022

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Colon tissue
• Species: Mouse
• Lot: SD206-7

Bioinformatics

• Gene Symbol: MAP2K1