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MEK1 [p Ser298] Antibody (SD206-7)

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Western Blot: MEK1 [p Ser298] Antibody (SD206-7) [NBP2-67770] - Analysis of Phospho-MEK1 (S298) on different lysates using anti-Phospho-MEK1 (S298) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: A431
Western Blot: MEK1 [p Ser298] Antibody (SD206-7) [NBP2-67770] - Analysis of Phospho-MEK1 (S298) on different lysates using anti-Phospho-MEK1 (S298) antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: MEK1 [p Ser298] Antibody (SD206-7) [NBP2-67770] - Staining Phospho-MEK1 (S298) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in ...read more
Immunohistochemistry-Paraffin: MEK1 [p Ser298] Antibody (SD206-7) [NBP2-67770] - Analysis of paraffin-embedded mouse colon tissue using MEK1 [p Ser298] antibody. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: MEK1 [p Ser298] Antibody (SD206-7) [NBP2-67770] - Staining Phospho-MEK1 (S298) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in ...read more
Immunocytochemistry/ Immunofluorescence: MEK1 [p Ser298] Antibody (SD206-7) [NBP2-67770] - Staining Phospho-MEK1 (S298) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in ...read more
Immunohistochemistry-Paraffin: MEK1 [p Ser298] Antibody (SD206-7) [NBP2-67770] - Analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-MEK1 (S298) antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
SD206-7
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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MEK1 [p Ser298] Antibody (SD206-7) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser298 of human MEK1. (SwissProt: Q02750 Human ;SwissProt: P31938 Mouse ;SwissProt: Q01986 Rat)
Modification
p Ser298
Localization
Cytoplasm, Nucleus, Membrane.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
MAP2K1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000
Reviewed Applications
Read 1 Review rated 5
using
NBP2-67770 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for MEK1 [p Ser298] Antibody (SD206-7)

  • dual specificity mitogen-activated protein kinase kinase 1
  • ERK activator kinase 1
  • MAP kinase kinase 1
  • MAP2K1
  • MAPK/ERK kinase 1
  • MAPKK1
  • MEK1
  • MEK1MAPKK 1
  • mitogen-activated protein kinase kinase 1
  • MKK1
  • MKK1MEK 1
  • PRKMK1EC 2.7.12.2
  • protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: ERK2 Antibody (SZ25-01) [NBP2-67360] - Staining ERK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line and MBA‑MB‑468 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for EphB2 at approximately 139 and 146 kDa (as indicated) using 20 µg/mL of Goat Anti-Human/Mouse EphB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF467) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Immunohistochemistry-Paraffin: ZHX2 Antibody [NBP1-82956] - Staining of human lymph node.
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Western Blot: Crk Antibody (3G11E8) [NBP2-37568] - Western blot analysis using CRK mAb against human CRK (AA: 1-204) recombinant protein. (Expected MW is 48.4 kDa)
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(6 Publications)
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Immunohistochemistry-Paraffin: AIMP2 Antibody [NBP1-81575] - Staining of human cerebral cortex shows weak to moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: AIMP2 Antibody [NBP1-81575] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.
Species: Hu, Mu, Rt
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MEK1 [p Ser298]
Antibody (SD206-7)
NBP2-67770
MEK1 [p Ser298] Antibody (SD206-7)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

Publications for MEK1 Antibody (NBP2-67770) (0)

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Review for MEK1 Antibody (NBP2-67770) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-67770:
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Immunohistochemistry-Paraffin MEK1 NBP2-67770
Enlarge 		5
reviewed by:
Verified Customer
 IHC-P Mouse 03/11/2022
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedColon tissue
SpeciesMouse
LotSD206-7

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Immunohistochemistry-Paraffin MEK1 NBP2-67770
Enlarge
 Verified Customer
03/11/2022
Application: IHC-P
Species: Mouse
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Bioinformatics

Gene Symbol MAP2K1