MED7 Overexpression Lysate (Native)

Images

 
Western Blot: MED7 Overexpression Lysate (Adult Normal) [NBL1-12994] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MED7.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MED7 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202696 and based on accession number NM_004270. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens mediator complex subunit 7 (MED7), transcript variant 2, mRNA.
Gene
MED7

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
27.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MED7 Overexpression Lysate (Native)

  • Activator-recruited cofactor 34 kDa component
  • Cofactor required for Sp1 transcriptional activation subunit 9
  • cofactor required for Sp1 transcriptional activation, subunit 9 (33kD)
  • cofactor required for Sp1 transcriptional activation, subunit 9, 33kDa
  • CRSP33CRSP complex subunit 9
  • CRSP9hMED7
  • mediator complex subunit 7ARC34
  • mediator of RNA polymerase II transcription subunit 7
  • MGC12284
  • RNA polymerase transcriptional regulation mediator subunit 7 homolog
  • Transcriptional coactivator CRSP33

Background

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Two transcript variants encoding the same protein have been found for this gene. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants encode the same protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol MED7