MED4 Antibody [Janelia Fluor® 585]

Product Discontinued

MED4 Antibody [Janelia Fluor® 585] Summary

• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human MED4. The immunogen is located within the last 50 amino acids of MED4. Amino Acid Squence: PGHNKEDEDDVEIMSTD
• Specificity: At least three isoforms of MED4 are known to exist; this antibody only recognizes the largest isoform.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MED4
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (86%)

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Peptide affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for MED4 Antibody [Janelia Fluor® 585]

• Activator-recruited cofactor 36 kDa component
• ARC36
• DRIP36
• DRIP36mediator of RNA polymerase II transcription, subunit 4 homolog (S. cerevisiae)
• FLJ10956
• HSPC126
• HSPC126vitamin D receptor interacting protein
• MED4
• mediator complex subunit 4TRAP/SMCC/PC2 subunit p36 subunit
• subunit 4 homolog
• TRAP36
• VDRIP

Background

MED4 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymera

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MED4