MED26 293T Cell Transient Overexpression Lysate

Product Discontinued

MED26 293T Cell Transient Overexpression Lysate Summary

• Description: Quality control test: Transient overexpression cell lysate was tested with Anti-DFFA antibody by Western Blots. Plasmid: pCMV-CRSP7 full-length
• Specificity: CRSP7 293T Cell Transient Overexpression Lysate(Denatured)
• Gene: MED26

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Lysate Details for Array

• Type: 293T Cell Transient Overexpression
• Protein State: Denatured

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MED26 293T Cell Transient Overexpression Lysate

• Activator-recruited cofactor 70 kDa component
• Cofactor required for Sp1 transcriptional activation subunit 7
• cofactor required for Sp1 transcriptional activation, subunit 7 (70kD)
• cofactor required for Sp1 transcriptional activation, subunit 7, 70kDa
• CRSP70ARC70
• CRSP7CRSP complex subunit 7
• mediator complex subunit 26Transcriptional coactivator CRSP70
• mediator of RNA polymerase II transcription subunit 26

Background

In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signals between transcriptional activators and RNA polymerase. These complexes include CRSP (for cofactor required for Sp1 activation), which is required, in conjunction with TAFIIs, for transcriptional activation by Sp1. CRSP is ubiquitously expressed in various tissues and functions as a multimeric complex that consists of nine distinct subunits. Several members of the CRSP family share sequence similarity with multiple components of the yeast transcriptional mediator proteins, including CRSP150, which is related to yeast Rgr1, and CRSP70, which is similar to the elongation factor TFIIS. CRSP77 and CRSP150 are also related to proteins within the putative murine mediator complex, while CRSP130 and CRSP34 are largely unrelated to either murine or yeast proteins. CRSP subunits also associate with larger multimeric coactivaor complexes, including ARC/DRI, which binds directly to SREBP and nuclear hormone receptors to facilitate transcription, and with NAT, a polymerase II-interacting complex that represses activated transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: MED26