MED16 Antibody (2B7) - Azide and BSA Free

Western Blot: MED16 Antibody (2B7) [H00010025-M02] - THRAP5 monoclonal antibody (M02), clone 2B7. Analysis of MED16 expression in A-431.

Immunocytochemistry/Immunofluorescence: MED16 Antibody (2B7) [H00010025-M02] - Analysis of monoclonal antibody to THRAP5 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: MED16 Antibody (2B7) [H00010025-M02] - Western Blot analysis of THRAP5 expression in Hela NE ( Cat # L013V3 ).

Western Blot: MED16 Antibody (2B7) [H00010025-M02] - THRAP5 monoclonal antibody (M02), clone 2B7. Analysis of MED16 expression in PC-12.

Western Blot: MED16 Antibody (2B7) [H00010025-M02] - THRAP5 monoclonal antibody (M02), clone 2B7. Analysis of MED16 expression in NIH/3T3.

Sandwich ELISA: MED16 Antibody (2B7) [H00010025-M02] - Detection limit for recombinant GST tagged THRAP5 is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clone: 2B7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MED16 Antibody (2B7) - Azide and BSA Free Summary

• Immunogen: THRAP5 (AAH17282, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MCDLRRPAAGGMMDLAYVCEWEKWSKSTHCPSVPLACAWSCRNLIAFTMDLRSDDQDLTRMIHILDTEHPWDLHSIPSEHHEAITCLEWDQSGSRLLSADADGQIKCWSM
• Specificity: THRAP5 - thyroid hormone receptor associated protein 5 (2B7)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MED16
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MED16 Antibody (2B7) - Azide and BSA Free

• DRIP92thyroid hormone receptor associated protein 5
• mediator complex subunit 16THRAP5
• mediator of RNA polymerase II transcription subunit 16
• Thyroid hormone receptor-associated protein 5
• Thyroid hormone receptor-associated protein complex 95 kDa component
• thyroid hormone receptor-associated protein, 95-kD subunit
• TRAP95
• Vitamin D3 receptor-interacting protein complex 92 kDa component

Background

MED16 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymera

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MED16
• Entrez:
  • 10025(Human)
• Uniprot:
  • AAH17282(Human)