MDM2/HDM2 Antibody

Western Blot: MDM2/HDM2 Antibody [NBP1-67627] - Analysis of lysates from SKOV3 cells, using MDM2 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunocytochemistry/Immunofluorescence: MDM2/HDM2 Antibody [NBP1-67627] - Analysis of A549 cells, using MDM2 Antibody. The picture on the right is blocked with the synthesized peptide.

Immunohistochemistry: MDM2/HDM2 Antibody [NBP1-67627] - Analysis of paraffin-embedded human breast carcinoma tissue, using MDM2 Antibody. The picture on the right isblocked with the synthesized peptide.

Product Discontinued

MDM2/HDM2 Antibody Summary

• Immunogen: The antiserum was produced against synthesized peptide derived from human MDM2. The immunogen region is: C-terminal.
• Specificity: MDM2 Antibody detects endogenous levels of total MDM2 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MDM2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:4000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MDM2/HDM2 Antibody

• ACTFS
• HDM2
• HDMX
• MDM2 oncogene, E3 ubiquitin protein ligase
• MDM2

Background

p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wt p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. These findings argue that MDM2 overexpression represents at least one mechanism by which p53 function can be abrogated during tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for MDM2/HDM2 Antibody (NBP1-67627) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anatoli Grechov WB Human 07/02/2014

Summary

• Application: Western Blot
• Sample Tested: 293T cell lysate
• Species: Human

FAQs for MDM2/HDM2 Antibody (NBP1-67627). (Showing 1 - 1 of 1 FAQ).

1. Hello, I am considering buying one of your rabbit (host) MDM2 antibodies for use in IFA. Based on the literature, there has been considerable difficulty in successfully labeling this protein in tissue. Does Novus have a return/replacement/refund policy for antibodies?
   • NBP1-67627 has been validated and is guaranteed to work in human and mouse for ELISA, Western blot, Immunofluorescence and IHC-Paraffin. Our 100% Novus Guarantee eliminates any risk to the researcher. Should you choose to use our antibody and encounter any troubles using the validated applications and species, we will be happy to troubleshoot with you. Should troubleshooting not be enough to resolve any potential problems, we would issue a refund.

Bioinformatics

• Gene Symbol: MDM2
• Entrez:
  • 4193(Human)
• OMIM:
  • 164785(Human)
• Uniprot:
  • Q00987()