Human Matriptase/ST14 ELISA Kit (Colorimetric)

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Human Matriptase/ST14 ELISA Kit (Colorimetric) [NBP3-07907] - Standard Curve

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP3-07907
    • Availability
      Product Discontinued

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Human Matriptase/ST14 ELISA Kit (Colorimetric) Summary

Specificity
No cross-reactivity with other serine proteases was detected for this assay.
Standard Curve Range
5-280 ng/ml
Sensitivity
1 ng/ml
Assay Type
Sandwich ELISA
Intra-Assay
CV% < 10%
Sample Volume
50 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
ST14

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Store at 4C.

Kit Components

Components
  1. 10X Streptavidin-HRP
  2. 10X Wash Buffer Concentrate
  3. Biotin-labeled monoclonal anti-Matriptase antibody
  4. Calibrators 1 and 2
  5. Matriptase reference standards
  6. Microtiter Plate
  7. Stop Solution
  8. TMB Reagent

Alternate Names for Human Matriptase/ST14 ELISA Kit (Colorimetric)

  • EC 3.4.21
  • Epithin
  • HAI
  • Matriptase
  • Membrane-type serine protease 1
  • MTSP1
  • MT-SP1EC 3.4.21.109
  • prostamin
  • PRSS14
  • Serine protease 14
  • Serine protease TADG-15
  • SNC19
  • SNC19MTSP1
  • ST14
  • suppression of tumorigenicity 14 (colon carcinoma)
  • suppression of tumorigenicity 14 (colon carcinoma, matriptase, epithin)
  • suppressor of tumorigenicity 14 protein
  • TADG15
  • TADG-15
  • TMPRSS14
  • tumor associated differentially expressed gene 15 protein
  • Tumor-associated differentially-expressed gene 15 protein

Background

ST14 is encoded by this gene is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion, and metastasis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Matriptase/ST14 ELISA Kit (NBP3-07907). (Showing 1 - 1 of 1 FAQ).

  1. What is the difference between the Quantikine and Duoset ELISA kits?
    • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

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Bioinformatics

Gene Symbol ST14